Bovine herpesvirus-1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is a major feedlot pathogen, which can cause substantial economic losses to the cattle industry. As BHV-1 can infect a number of tissues in the body, there are five distinct disease syndromes. In addition to causing respiratory disease, BHV-1 can cause conjunctivitis (red eye), infectious pustular vulvovaginitis or balanoposthitis (IPV; genital disease in both females and males), abortions, and encephalitis (brain infections). BoHV-1 is distributed worldwide. This virus may be transmitted via respiratory and venereal routes and by artificial insemination. Although all ages and breeds of cattle are susceptible to IBR, the disease is most common in calves over six months of age. Diagnosis of uncomplicated IBR can usually be made based on the clinical signs, pattern of onset and lesions. However, this diagnosis should be confirmed by laboratory tests. Molecular detection of BHV-1 by real-time PCR is more rapid, accurate and convenient method with quantitative result and high sample throughput.