BRAF, a kinase-encoding gene from the RAS/RAF/MAPK pathway is recently being studied in relation to prognosis of colorectal cancer.
Ninety percent of the BRAF mutations consist V600E mutation (1799T>A) which causes the constitutive activation of the protein.The presence of BRAF mutation is significantly associated with right-sided colon cancers and is associated with decreased overall survival.
BRAF mutations were suggested to be a negative predictor of response to anti-EGFR therapy.
BRAF SNP screening real-time PCR kit is a multiplex real-time PCR which uses a pair of primer with two fluorogenic probes based chemistry that uses the 5 ́ nuclease activity of Taq DNA polymerase.
BRAF mutation screening kit detects single nucleotide polymorphism (SNP) – 1799T>A responsible for mutation V600E and discriminates mutant allele from wild type in a single tube.The kit includes all the reagents from sample to SNP detection.
A combination of robust reagents for DNA extraction from clinical samples and validated primers, hydrolysis probes as well as hot start Taq polymerase for SNP detection ensures that kit leads to highly specific and sensitive results in short time.
|#||Product||Cat. No.||Reaction size/kit|
|1||Oncoscreen BRAF Mutation Detection Kit (V600E)||OBMD25||25|
|2||Oncoscreen BRAF Mutation Detection Kit (V600E)||OBMD50||50|