EGFR gene in non-small-cell lung cancer (NSCLC) is predictive of response to treatment with tyrosine kinase inhibitors (TKIs)].These mutations are usually found in exons 18 through 21 of the TK domain of the EGFR and are either point mutations or in-frame small deletions or insertions.
Although more than 250 mutations of the EGFR have been described up to now, two variants, a single point mutation in exon 21, the L858R and exon 20 T790M in respectively account for 50% of all EGFR mutations.
In order to determine whether an EGFR TKI or chemotherapy is the appropriate first line therapy, guidelines recommend mutation testing for all patients with advanced NSCLC tumor and adenocarcinoma histology.
EGFR SNP screening real-time PCR kit is a multiplex real-time PCR which uses a pair of primer with two fluorogenic probes based chemistry that uses the 5 ́ nuclease activity of Taq DNA polymerase.
This protocol is successfully used to detect single nucleotide polymorphism (SNP) which discriminates mutant allele from wild type in a single tube. In EGFR mutation screening test, two reactions per sample is performed for detection of utation L858R (2573T>G) and T790M (2369C>T). The kit includes all the reagents from sample to SNP detection.
A combination of robust reagents for DNA extraction from clinical samples and validated primers, hydrolysis probes as well as hot start Taq polymerase for SNP detection ensures that kit leads to highly specific and sensitive results in short
| # | Product | Cat. No. | Reaction size/kit |
|---|---|---|---|
| 1 | Oncoscreen EGFR Mutation Detection kit (L858R & T790M) | OED25 | 25 |
| 2 | Oncoscreen EGFR Mutation Detection kit (L858R & T790M) | OED50 | 50 |
