Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii, the most common parasitic infection worldwide affecting animals as well as humans. The most common symptoms of the disease are anorexia, weight loss, lethargy, dyspnea, ocular symptoms and pyrexia. As toxoplasmosis can be transmitted to humans it represents a serious health risk for people living in close contact with infected animals. Infection is especially dangerous for people with suppressed immune systems and cancer patients as well as pregnant women.
Routine diagnosis is based on serology. A rapid and accurate diagnosis is required to initiate treatment. Serological diagnosis of active infection is unreliable because reactivation is not always accompanied by changes in antibody levels, and the presence of IgM does not necessarily indicate recent infection. Application of qualitative real time PCR has evolved as a sensitive, specific and rapid method for the detection of Toxoplasma gondii DNA in amniotic fluid, blood, tissue samples and cerebrospinal fluids.
Mylab Lifesolutions provides ready to use solution for detection of Toxoplasma gondii using real time PCR technology. Detection is based on the amplification of a specific conservative DNA sequence of Toxoplasma gondii and detection by Taqman probe based chemistry. An Internal control is detected along with the CMV target controlling the extraction efficacy and possible PCR inhibition.
The test is designed to use across wide range of PCR platforms. A combination of validated primers, hydrolysis probes, and hot-start DNA polymerase ensures that the kit leads to highly-specific and ultrasensitive results in short time.
|PathoDetect Toxoplasmosis Detection kit