Tuberculosis remains a major global health problem and ranks as the second leading cause of death from an infectious disease worldwide, after HIV. It is the first infectious disease declared by the World Health Organization (WHO) as a global health emergency.The diagnosis of M. tuberculosis complex is required for the definitive diagnosis of tuberculosis and optimal control.

The conventional identification of MTB isolates by culture and phenotypic characterization is widely used but it takes 4 to 6 weeks or longer as for slow growing species. Molecular genetic approaches for M. tuberculosis identification directly from clinical samples or on cultured isolates are preferred because of their high sensitivity and specificity, as well as rapid processing time.

Mycobacteria cause a variety of illnesses, including tuberculosis, which has profound individual and public health implications. The identification and differentiation of Mycobacterium tuberculosis (MTB) from non tuberculous mycobacteria (NTM) has important ramifications for infection control and the selection of antimicrobial therapy.

Mylablifesolutions designed multiplex real-time PCR assay for simultaneous detection of all mycobacteria and further discrimination of M. tuberculosis (MTB) and nontuberculous mycobacteria (NTM) from clinical samples.